Association of Interleukin-18 Gene Polymorphism with Susceptibility to Visceral Leishmaniasis in Endemic Area of Bihar, an Indian Population

Table 2

Polymerase chain reaction (PCR) primers and condition for IL-18 gene amplification.

SNPs	PCR primers	Annealing temperature (°C)	Restriction enzymes	Fragment sizes (bp)
+105; (A/C)	F: AGATTTAATGTTTATTGTAGAAAACCTGGGACTC; R: CAGTCATATCTTCAAATAGAGGCCG	55	Dde I	A: 109 + 32; C: 141
−656; (G/T)	F: AGGTCAGTCTTTGCTATCATTCCAGG; R: CTGCAACAGAAAGTAAGCTTGCGGAGAGG	60	Mwo I	G: 96 + 24; T: 120
−137; (G/C)	F: CACAGAGCCCCAACTTTTTACGGCAGAGAA; R: GACTGCTGTCGGCACTCCTTGG	60	Mbo II	G: 116 + 39; C: 155