Phytoestrogen α-Zearalanol Improves Memory Impairment and Hippocampal Neurogenesis in Ovariectomized Mice
(a) Photomicrograph of 5-bromo-2-deoxyuridine (BrdU) immunopositive cells (new born neurons) in DG in the hippocampus of control and OVX mice with 17β-E2 or α-ZAL treatment. Fewer BrdU-labeled cells were observed in OVX mice compared to control. 17β-E2 and α-ZAL-treated mice expressed higher BrdU+ cells compared to OVX animal. Magnification ×40. (b) Quantification of BrdU immunolabeling cells in DG. in each group and the result indicated at least three independent experiments in each animal. versus control group; versus OVX group.