Culture and Characterization of Microglia from the Adult Murine Retina
Figure 2
Morphology and proliferation of the cultured microglial cells in different percentages of the L929 conditioned supernatant. (a) Mixed retinal culture grown in 0% L929 supernatant yielded few cells. (b) 10% and (c) 20% of L929 conditioned supernatant produced the highest yield. (d) 30% L929 conditioned supernatant produced less yield compared to 20%. Microglial cells were observed using a phase-contrast Olympus microscope and Prog Res C14 imaging software. Magnification ×20. Data are representative of 3 experimental repeats.
