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The Scientific World Journal
Volume 2014, Article ID 895167, 6 pages
Research Article

Studies on Optimization of Growth Parameters for L-Asparaginase Production by Streptomyces ginsengisoli

Department of Microbiology, Abasaheb Garware College, Pune 411004, India

Received 31 August 2013; Accepted 10 December 2013; Published 29 January 2014

Academic Editors: A. Corsetti, A. J. Piantino Ferreira, and J. Yoon

Copyright © 2014 Neelima Deshpande et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A species of Streptomyces, Streptomyces ginsengisoli, a river isolate, was evaluated for production of an enzyme, L-asparaginase, with multiple functions mainly anticancer activity. The actinomycete was subjected to submerged fermentation by “shake flask” method. The quantity of L-asparaginase produced was estimated as 3.23 μmol/mL/min. The effect of various culture conditions on L-asparaginase production was studied by adopting a method of variation in one factor at a time. Of the various conditions tested, glucose (followed by starch) and peptone served as good carbon and nitrogen sources, respectively, for maximal production of enzyme at pH 8. The temperature of 30°C and an incubation period of 5 days with 0.05 g% asparagine concentration were found to be optimum for L-asparaginase production.