Pyruvic Oxime Nitrification and Copper and Nickel Resistance by a Cupriavidus pauculus, an Active Heterotrophic Nitrifier-Denitrifier

Table 1

Growth and nitrification of PO by C. pauculus UM1 in the presence of either 1 mM Cu²⁺ or 0.5 mM Ni²⁺ in either NB or PO-mineral salts medium^a,b.

Growth condition	Time (d)
	0	7	0	7
	Growth (Abs600)	Growth (Abs600)	(mM)	(mM)
NB-1 mM Cu^2+	0.01 (0.00)	2.04 (0.09)	0.00 (0.00)	0.01 (0.02)
NB-1 mM Cu^2+-PO	0.07 (0.00)	2.50 (0.26)	0.00 (0.00)	13.62^d (0.48)
NB-0.5 mM Ni^2+	0.00 (0.00)	1.77 (0.04)	0.00 (0.00)	0.03 (0.01)
NB-0.5 mM Ni^2+-PO	0.01 (0.00)	2.07^d (0.06)	0.00 (0.00)	6.61^d (1.29)
PO-1 mM Cu^2+ (uninoculated)	0.01 (0.01)	0.00 (0.00)	0.00 (0.00)	−0.04 (0.00)
PO-1 mM Cu^2+	0.00 (0.00)	0.14^d (0.01)	0.03^d (0.01)	10.39^d (0.32)
PO-0.5 mM Ni^2+ (uninoculated)	0.00 (0.00)	0.00 (0.00)	−0.01 (0.01)	−0.01 (0.00)
PO-0.5 mM Ni^2+	0.00 (0.00)	0.23^c (0.06)	0.12^d (0.01)	12.36^d (0.98)

Inocula for the NB-1 mM Cu²⁺ and the NB-0.5 mM Ni²⁺ experiments were grown in the NB-1 mM Cu²⁺ and NB-0.5 mM Ni²⁺ medium, respectively, until late logarithmic growth and used at 0.5% (v/v). Inocula for the PO-1.0 mM Cu²⁺ medium and for the PO-0.5 mM Ni²⁺ medium were grown in 0.2% PO medium until late logarithmic growth and used at 0.5% (v/v).
^bStandard deviation values are in parentheses.
^cStatistically different from the corresponding control at 0.05.
^dStatistically different from the corresponding control at 0.01.