Pyruvic Oxime Nitrification and Copper and Nickel Resistance by a Cupriavidus pauculus, an Active Heterotrophic Nitrifier-Denitrifier

Table 2

Growth of C. pauculus UM1 when in the presence of either no metals, 1 mM Cu²⁺, or 0.5 mM Ni²⁺ in the phenol-mineral salts medium^a,b.

Growth condition	Time (d)
	0	3	6	0	3	6
	Growth (Abs600)	Growth (Abs600)	Growth (Abs600)	Phenol (mM)	Phenol (mM)	Phenol (mM)
Phenol	0.0018 (0.0005)	0.0800^d (0.0028)	0.0715^d,e (0.0038)	1.89 (0.12)	0.27^d (0.01)	0.20^d,e (0.01)
Phenol with 1 mM Cu^2+	0.0920 (0.0094)	0.1417^d (0.0118)	0.1385 (0.0324)	1.89 (0.12)	0.27^d (0.02)	0.22^d,e (0.00)
Phenol with 0.5 mM Ni^2+	0.0025 (0.0005)	0.0769^c (0.0252)	0.1793^c (0.0616)	1.93 (0.18)	1.30 (0.57)	0.20^d (0.00)

Inocula were a 1 : 100 dilution of C. pauculus UM1 grown to midlogarithmic phase in 2 mM phenol-mineral salts medium. The relatively large = 0 Abs₆₀₀ value of the phenol with 1 mM Cu²⁺ cultures was due to the blue color imparted to the medium by Cu²⁺.
^bStandard deviation values are in parentheses.
^cStatistically different from the initial (time 0) measurement at 0.05.
^dStatistically different from the initial (time 0) measurement at 0.01.
^eStatistically different from the day 3 measurement at 0.05.