RHBDL2 Is a Critical Membrane Protease for Anoikis Resistance in Human Malignant Epithelial Cells
Figure 1
RHBDL2 overexpression leads to promotion of cell proliferation, reduction of adhesion ability, and suppression of homeless cell death in human keratinocytes. (a) The illustrated RHBDL2 expression construct and the control vector and relative protein expression levels in stable transfected HaCaT cells. (b) Proliferation of the two stable transfected HaCaT cells was measured using the WST-1 assay. (c) Adhesive ability of the different transfected cells was quantified by type I collagen adhesion assay. (d) Activation of caspase 3 under floating culture condition in stable transfected cells was investigated by Western blotting. (e) Quantification of apoptotic cells in stable cells floating for 24 hours by using Annexin V-FITC/PI staining. ; .
