RHBDL2 Is a Critical Membrane Protease for Anoikis Resistance in Human Malignant Epithelial Cells
Figure 3
RHBDL2 is essential for anoikis resistance in HeLa S3 cells. (a) Western blot analysis revealed the protein levels of cleaved caspase 3 in suspension-cultured HeLa S3 cells treated with various doses of DCI for 48 hours. (b) Silenced RNA level of RHBDL2 by shRHBDL2 construct was detected by RT-PCR. (c) The protein expression of RHBDL2 and cleaved caspase 3 in shRHBDL2-transfected cells were estimated by Western blotting. (d) Quantification of apoptotic cells under floating culture condition by using Annexin V-FITC/PI staining.
