Biocompatibility and Toxicity of Poly(vinyl alcohol)/N,O-Carboxymethyl Chitosan Scaffold

Figure 3

Immunohistology staining for CD 68 of the PVA hydrogel and HAM after 5, 10, and 15 days of subcutaneous implantation, 400x. Briefly, the samples were rinsed with phosphate buffered saline (PBS) and fixed with methanol for 15 min. Then, the samples were treated with 0.03% hydrogen peroxide for 5 min and incubated with mouse anti-rabbit anti-CD 68 antibody for 30 min at 1 : 100 dilutions and then with peroxidase-labeled polymer conjugated to goat anti-mouse immunoglobulin for another 30 min. The expression of CD 68 has been indicated by arrow (100x).