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The Scientific World Journal
Volume 2014, Article ID 916713, 5 pages
Research Article

In Vitro Vitamin K3 Effect on Conjunctival Fibroblast Migration and Proliferation

1Department of Ophthalmology, Lozano Blesa University Hospital, C/San Juan Bosco 15, 50009 Zaragoza, Spain
2Aragon Institute of Health Sciences (IIS Aragon), 50009 Zaragoza, Spain
3Department of Ophthalmology, Hospital García Orcoyen, Navarra, 31200 Estella, Spain
4Department of Ophthalmology, Complejo Hospitalario de Navarra, Navarra, 31008 Pamplona, Spain
5Department of Applied Physics, Zaragoza University, 50009 Zaragoza, Spain
6Department of Gastroenterology, Lozano Blesa University Hospital, C/San Juan Bosco 15, 50009 Zaragoza, Spain

Received 29 August 2013; Accepted 14 October 2013; Published 8 January 2014

Academic Editors: J. Aquavella and K. Unlu

Copyright © 2014 I. Pinilla et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. To evaluate the dose effect of vitamin K3 on wound healing mechanisms. Methods. Conjunctival fibroblasts were incubated for 24 hours. An artificial wound was made and the cells were incubated with fresh medium plus doses of vitamin K3 to be tested. Wound repair was monitored at 0, 18, 24, and 48 hours. Proliferation was measured in actively dividing cells by [3H]thymidine uptake. Six different groups were tested: group 1/no drugs added, group 2/ethanol 0.1%, group 3/vitamin K3 1 mg/L, group 4/vitamin K3 2 mg/L, group 5/vitamin K3 4 mg/L, and group 6/vitamin K3 6 mg/L. Each experiment was carried out in triplicate and 4 times. Results. There were no differences among groups at the initial time. In vitro wound repair was slower in groups 4, 5, and 6. There were no differences between control and ethanol groups and between control and vitamin K3 1 mg/L groups. Fibroblast mitogenic activity was statistically decreased in all vitamin K groups; statistical differences were found among vitamin K3 1 mg/mL and higher doses too. In groups 5 and 6, cellular toxicity was presented. Conclusions. Vitamin K3 is able to inhibit fibroblast proliferation. Vitamin K3 2 mg/L or higher doses inhibit wound healing repair, exhibiting cellular toxicity at 4 and 6 mg/L.