Development of Strand-Specific Real-Time RT-PCR to Distinguish Viral RNAs during Newcastle Disease Virus Infection

Figure 3

Selection of the optimal adsorption time for La Sota infection. gRNA, cRNA, and mRNA levels were measured at 5 s, 1 min, 5 min, 10 min, 15 min, 20 min, 25 min, and 30 min after DF-1 cells were infected with the La Sota strain at an MOI of 1. The optimal adsorption time was selected as 20 min, since the attachment and detachment of La Sota were balanced at this time point.