Development of Strand-Specific Real-Time RT-PCR to Distinguish Viral RNAs during Newcastle Disease Virus Infection

<div>Kinetics of gRNA, cRNA, and mRNA in La Sota infected DF-1 cells. DF1 cells were incubated with NDV La Sota at an MOI of 1 for 20 min. The gRNA, cRNA, and mRNA were quantitatively analyzed at 1, 2, 3, 4, 5, 6, 8, 10, 12, 16, 20, 24, 36, and 48 hpi. (a) Kinetics of gRNA and cRNA. (b) Kinetics of NP gene mRNA.</div>

The Scientific World Journal

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Figure 4: Development of Strand-Specific Real-Time RT-PCR to Distinguish Viral RNAs during Newcastle Disease Virus Infection 