The Scientific World Journal

Research Article

Development of Strand-Specific Real-Time RT-PCR to Distinguish Viral RNAs during Newcastle Disease Virus Infection

Table 1

Primers used in this study.
PrimersSequences (5′-3′)Location3Fragment designation
Pla1F1TATCCAGGCTCAAGTATGGGTCACA622–646Pla1
Pla1R2CCTTGGTCTTGCCTTGTGGGATTG1999–2022
Pla2FATATTCAGAGATCAGGGCAAGTC1819–1841Pla2
Pla2RGTTTGCCACAACCCTACAGC3708–3724
Pla3FAAAGCTGTAGGGTTGTGG3705–3727Pla3
Pla3RTTGGCGATGACTGAACCT 5704–5721
Pla4FAGGCGCACTTACTACACCATA 5671–5691Pla4
Pla4RTGCTGTATGCGTTTCCCACCA 7502–7525
Pla5FTACAGCAGGCTATCTTATCT 7517–7536Pla5
Pla5RTGAGTCACGGATTCTGCT 9370–9387
Pla6FGTGACTCATGCAATCGCTACT 9377–9400Pla6
Pla6RCTAATTGGGCAGGAGTCAGA 11106–11126
Pla7FTGCAGAGATCAAGCGACTA 11197–11215Pla7
Pla7RAGCATCTTCTCATTCAGGTTATC 13105–13128
Pla8FTATCCCGGTTATGCTGTC 13137–13154Pla8
Pla8RTAAGACATTTATTTGAGTTCG 15103–15122
Pla-rt11CAATAGGAGTGGAGTGTCTGA466–486Real time
Pla-rt12TCCTCTCCAGGGTATCGGTGA549–614
Pla-rt13CAACAATAGGAGTGGAGTGTCTGA463–486Real time
Pla-rt14CAGGGTATCGGTGATGTCTTCT586–607
Pla-rt21TCTGTACTGTACTTGACTCGTGCTC14903–14927Real time
Pla-rt22CTGTAATATCCGTTGACTGCATTGCCT14953–14979
1F represents forward primer.
2R represents reverse transcription- (RT-) primer.
3The location was based on the genomic sequence of NDV La Sota (GenBank ID: LaSota_AF077761).