Full-Length Sequence Analysis of Chloroquine Resistance Transporter Gene in Plasmodium falciparum Isolates from Sabah, Malaysia

Table 1

Primers for pfcrt PCR amplification.

Primer	Primer sequence	Expected size (bp)	Reference
First fragment
E1/2-F; E1/2-R	5′-CGACATTCCGATATATTATATTTTAGAC-3′; 5′-TATATGTGTAATGTTTTATATTGG-3′	740	[14]
E1/2-NF; E1/2-NR	5′-CCGTTAATAATAAATACACGCAG-3′; 5′-AATGTTTTATATTGGTAGGTGG-3′	694
Second fragment
E3/8-F; E3/8-R	5′-CCACCTACCAATATAAAACATTAC-3′; 5′-GTTAAAATATATATAAATGTCTC-3′	1446
E3/8-NF	5′-TATATATATATGTATGTATGTTG-3′	1370
E3/8-NR	5′-AATGTCTCTTATAATTTTGAAATT-3′
Third fragment
E9/13-F	5′-CTTATAATAAAATTTCAAAATTATAAGAGAC-3′	1287
E9/13-R	5′-GAGATCTCTATACCTTCAACATTATTCC-3′
E9/13-NF; E9/13-NR	5′-GAGACATTTATATATATTTTAAC-3′; 5′-CCTTATAAAGTGTAATGCG-3′	1235