The In Vitro Effect of Ivermectin on the Activity of Trehalose Synthesis Pathway Enzymes and Their mRNA Expression in the Muscle of Adult Female Ascaris suum (Nematoda)
Table 2
Activity of trehalose-6-phosphate synthase (TPS) and phosphatase (TPP) and their mRNA expression (tps1, tps2, and tpp genes) during in vitro cultivation of adult female Ascaris suum in control (C-group) and ivermectin-supplemented medium (IVM-group).
Group of worms
Activity of TPS [nM/mg]
Activity of TPS relative to the start [%]
tps1 RQ to the start
tps2 RQ to the start
Activity of TPS relative to the control [%]
tps1 RQ to the control
tps2 RQ to the control
S-group
100
1
1
—
—
—
4 h C-group
67.13 ± 2.56
100
1
1
8 h C-group
53.85 ± 2.03
100
1
1
20 h C-group
33.64 ± 3.07
100
1
1
Correlation coefficient
4 h IVM-group
54.12 ± 1.74
8 h IVM-group
64.64 ± 4.4
20 h IVM-group
31.13 ± 1.85
Correlation coefficient
Group of worms
Activity of TPP [nM/mg]
Activity of TPP relative to the start [%]
tpp RQ to the start
Activity of TPP relative to the control [%]
tpp RQ to the control
S-group
100
1
—
—
4 h C-group
63.48 ± 0.84
100
1
8 h C-group
45.97 ± 1.33
100
1
20 h C-group
41.13 ± 1.15
100
1
Correlation coefficient
4 h IVM-group
26.35 ± 0.74
8 h IVM-group
30.88 ± 1.85
20 h IVM-group
32.26 ± 2.18
Correlation coefficient
Pearson’s correlation coefficient between gene expression and appropriate enzyme activity changes over time with respect to the start group (S); **Pearson’s correlation coefficient between gene expression and appropriate enzyme activity changes over time with respect to the C-group.
