miR-16-1 Promotes the Aberrant α-Synuclein Accumulation in Parkinson Disease via Targeting Heat Shock Protein 70

Figure 3

miR-16-1 targets the 3′ UTR of Hsp70 and reduces Hsp70 expression in human neuroblastoma SH-SY5Y-Syn cell line. (a) miR-16-1/3′ UTR of Hsp70 alignment by miRanda analysis. (b) The manipulation of miR-16-1 level in SH-SY5Y-Syn cells. Cells were transfected with 25 or 50 nM miR-16-1 mimics or miR control; 24 h later, the miR-16-1 level was examined by RT-qPCR. (c) and (d) Hsp70 expression was suppressed in mRNA level (c) or in protein level ((d) and (e)) in SH-SY5Y-Syn cells. All experiments were performed in triplicate. No significance, ^∗𝑃<0.05, or ^∗∗∗𝑃<0.001.

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