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The Scientific World Journal
Volume 2014, Article ID 946394, 7 pages
http://dx.doi.org/10.1155/2014/946394
Research Article

Molecular Characterization of Enterohemorrhagic E. coli O157 Isolated from Animal Fecal and Food Samples in Eastern China

Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China

Received 14 February 2014; Revised 19 May 2014; Accepted 19 May 2014; Published 4 June 2014

Academic Editor: Dongsheng Zhou

Copyright © 2014 Shaohui Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. C. L. Gyles, “Shiga toxin-producing Escherichia coli: an overview,” Journal of Animal Science, vol. 85, no. 13, supplement, pp. E45–E62, 2007. View at Publisher · View at Google Scholar · View at Scopus
  2. A. Sugiyama, Y. Iwade, S. Akachi et al., “An outbreak of Shigatoxin-producing Escherichia coli O157:H7 in a nursery school in Mie Prefecture,” Japanese Journal of Infectious Diseases, vol. 58, no. 6, pp. 398–400, 2005. View at Google Scholar · View at Scopus
  3. Y. Xiong, P. Wang, R. Lan et al., “A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China,” PLoS ONE, vol. 7, no. 4, Article ID e36144, 2012. View at Publisher · View at Google Scholar · View at Scopus
  4. D. Law, “Virulence factors of Escherichia coli O157 and other Shiga toxin-producing E. coli,” Journal of Applied Microbiology, vol. 88, no. 5, pp. 729–745, 2000. View at Publisher · View at Google Scholar · View at Scopus
  5. C.-J. Wu, P.-R. Hsueh, and W.-C. Ko, “A new health threat in Europe: Shiga toxin-producing Escherichia coli O104:H4 infections,” Journal of Microbiology, Immunology and Infection, vol. 44, no. 5, pp. 390–393, 2011. View at Publisher · View at Google Scholar · View at Scopus
  6. R. M. Donlan and J. W. Costerton, “Biofilms: survival mechanisms of clinically relevant microorganisms,” Clinical Microbiology Reviews, vol. 15, no. 2, pp. 167–193, 2002. View at Publisher · View at Google Scholar · View at Scopus
  7. M. E. Davey and G. A. O'Toole, “Microbial biofilms: from ecology to molecular genetics,” Microbiology and Molecular Biology Reviews, vol. 64, no. 4, pp. 847–867, 2000. View at Publisher · View at Google Scholar · View at Scopus
  8. A. V. Tutenel, D. Pierard, J. Van Hoof, M. Cornelis, and L. De Zutter, “Isolation and molecular characterization of Escherichia coli O157 isolated from cattle, pigs and chickens at slaughter,” International Journal of Food Microbiology, vol. 84, no. 1, pp. 63–69, 2003. View at Publisher · View at Google Scholar · View at Scopus
  9. M. Maruzumi, M. Morita, Y. Matsuoka, A. Uekawa, T. Nakamura, and K. Fuji, “Mass food poisoning caused by beef offal contaminated by Escherichia coli O157,” Japanese Journal of Infectious Diseases, vol. 58, no. 6, p. 397, 2005. View at Google Scholar · View at Scopus
  10. W. A. Ferens and C. J. Hovde, “Escherichia coli O157:H7: animal reservoir and sources of human infection,” Foodborne Pathogens and Disease, vol. 8, no. 4, pp. 465–487, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. Y. Yan, Y. Shi, D. Cao, X. Meng, L. Xia, and J. Sun, “Prevalence of Stx phages in environments of a pig farm and lysogenic infection of the field E. coli O157 isolates with a recombinant converting phage,” Current Microbiology, vol. 62, no. 2, pp. 458–464, 2011. View at Publisher · View at Google Scholar · View at Scopus
  12. S. Radu, O. W. Ling, G. Rusul, M. I. A. Karim, and M. Nishibuchi, “Detection of Escherichia coli O157:H7 by multiplex PCR and their characterization by plasmid profiling, antimicrobial resistance, RAPD and PFGE analyses,” Journal of Microbiological Methods, vol. 46, no. 2, pp. 131–139, 2001. View at Publisher · View at Google Scholar · View at Scopus
  13. J.-Y. Kim, S.-H. Kim, N.-H. Kwon et al., “Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD,” Journal of Veterinary Science, vol. 6, no. 1, pp. 7–19, 2005. View at Google Scholar · View at Scopus
  14. S. Rajkhowa, J. Scaria, D. L. Garcia, K. A. Musser, B. L. Akey, and Y.-F. Chang, “Analysis of Escherichia coli O157 clinical isolates by multilocus sequence typing,” BMC Research Notes, vol. 3, article 343, 2010. View at Publisher · View at Google Scholar · View at Scopus
  15. X. W. Ji, Y. L. Liao, Y. F. Zhu et al., “Multilocus sequence typing and virulence factors analysis of Escherichia coli O157 strains in China,” Journal of Microbiology, vol. 48, no. 6, pp. 849–855, 2010. View at Publisher · View at Google Scholar · View at Scopus
  16. A. C. Noller, M. C. McEllistrem, O. C. Stine et al., “Multilocus sequence typing reveals a lack of diversity among Escherichia coli O157:H7 isolates that are distinct by pulsed-field gel electrophoresis,” Journal of Clinical Microbiology, vol. 41, no. 2, pp. 675–679, 2003. View at Publisher · View at Google Scholar · View at Scopus
  17. F. T. Biscola, C. M. Abe, and B. E. C. Guth, “Determination of adhesin gene sequences in, and biofilm formation by, O157 and non-O157 Shiga toxin-producing Escherichia coli strains isolated from different sources,” Applied and Environmental Microbiology, vol. 77, no. 7, pp. 2201–2208, 2011. View at Publisher · View at Google Scholar · View at Scopus
  18. J. R. Johnson, K. L. Owens, C. R. Clabots, S. J. Weissman, and S. B. Cannon, “Phylogenetic relationships among clonal groups of extraintestinal pathogenic Escherichia coli as assessed by multi-locus sequence analysis,” Microbes and Infection, vol. 8, no. 7, pp. 1702–1713, 2006. View at Publisher · View at Google Scholar · View at Scopus
  19. S. Sánchez, R. Martínez, A. García et al., “Shiga toxin-producing Escherichia coli O157:H7 from extensive cattle of the fighting bulls breed,” Research in Veterinary Science, vol. 88, no. 2, pp. 208–210, 2010. View at Publisher · View at Google Scholar · View at Scopus
  20. J. Bai, X. Shi, and T. G. Nagaraja, “A multiplex PCR procedure for the detection of six major virulence genes in Escherichia coli O157:H7,” Journal of Microbiological Methods, vol. 82, no. 1, pp. 85–89, 2010. View at Publisher · View at Google Scholar · View at Scopus
  21. S. Wang, C. Niu, Z. Shi et al., “Effects of ibeA deletion on virulence and biofilm formation of avian pathogenic Escherichia coli,” Infection and Immunity, vol. 79, no. 1, pp. 279–287, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. O. Clermont, S. Bonacorsi, and E. Bingen, “Rapid and simple determination of the Escherichia coli phylogenetic group,” Applied and Environmental Microbiology, vol. 66, no. 10, pp. 4555–4558, 2000. View at Publisher · View at Google Scholar · View at Scopus
  23. T. Wirth, D. Falush, R. Lan et al., “Sex and virulence in Escherichia coli: an evolutionary perspective,” Molecular Microbiology, vol. 60, no. 5, pp. 1136–1151, 2006. View at Publisher · View at Google Scholar · View at Scopus
  24. S.-K. Lim, H.-S. Lee, H.-M. Nam et al., “Antimicrobial resistance observed in Escherichia coli strains isolated from fecal samples of cattle and pigs in Korea during 2003-2004,” International Journal of Food Microbiology, vol. 116, no. 2, pp. 283–286, 2007. View at Publisher · View at Google Scholar · View at Scopus
  25. C. N. Ateba and M. Mbewe, “Detection of Escherichia coli O157:H7 virulence genes in isolates from beef, pork, water, human and animal species in the northwest province, South Africa: public health implications,” Research in Microbiology, vol. 162, no. 3, pp. 240–248, 2011. View at Publisher · View at Google Scholar · View at Scopus
  26. Y. Sasaki, Y. Tsujiyama, M. Kusukawa, M. M. S. Katayama, and Y. Yamada, “Prevalence and characterization of Shiga toxin-producing Escherichia coli O157 and O26 in beef farms,” Veterinary Microbiology, vol. 150, no. 1-2, pp. 140–145, 2011. View at Publisher · View at Google Scholar · View at Scopus
  27. J. Madic, N. Vingadassalon, C. P. de Garam et al., “Detection of Shiga toxin-producing Escherichia coli serotypes O26:H11, O103:H2, O111:H8, O145:H28, and O157:H7 in raw-milk cheeses by using multiplex real-time PCR,” Applied and Environmental Microbiology, vol. 77, no. 6, pp. 2035–2041, 2011. View at Publisher · View at Google Scholar · View at Scopus
  28. Z. Zhou, Y. Nishikawa, P. Zhu et al., “Isolation and characterization of Shiga toxin-producing Escherichia coli O157:H7 from beef, pork and cattle fecal samples in Changchun, China,” Journal of Veterinary Medical Science, vol. 64, no. 11, pp. 1041–1044, 2002. View at Publisher · View at Google Scholar · View at Scopus
  29. J. Wang, L.-P. Shen, P.-H. Liu et al., “Study on Escherichia coli O157:H7 among animal and animal's products in Shanghai,” Progress in Veterinary Medicine, vol. 26, no. 4, pp. 87–90, 2005. View at Google Scholar
  30. J.-G. Xu, B.-K. Cheng, and H.-Q. Jing, “Escherichia coli O157: H7 and Shiga-like-toxin-producing Escherichia coli in China,” World Journal of Gastroenterology, vol. 5, no. 3, pp. 191–194, 1999. View at Google Scholar · View at Scopus
  31. C. N. Ateba and C. C. Bezuidenhout, “Characterisation of Escherichia coli O157 strains from humans, cattle and pigs in the North-West Province, South Africa,” International Journal of Food Microbiology, vol. 128, no. 2, pp. 181–188, 2008. View at Publisher · View at Google Scholar · View at Scopus
  32. E. Şeker, Y. Kuyucuoǧlu, B. Sareyyüpoǧlu, and H. YardiMci, “PCR detection of shiga toxins, enterohaemolysin and intimin virulence genes of Escherichia coli O157: H7 strains isolated from faeces of anatolian water buffaloes in Turkey,” Zoonoses and Public Health, vol. 57, no. 7-8, pp. e33–e37, 2010. View at Publisher · View at Google Scholar · View at Scopus
  33. B. Swaminathan, T. J. Barrett, S. B. Hunter, and R. V. Tauxe, “PulseNet: the molecular subtyping network for foodborne bacterial disease surveillance, United States,” Emerging Infectious Diseases, vol. 7, no. 3, pp. 382–389, 2001. View at Google Scholar · View at Scopus