Characterization of Metabolites of Leonurine (SCM-198) in Rats after Oral Administration by Liquid Chromatography/Tandem Mass Spectrometry and NMR Spectrometry

Table 1

The metabolic profile of leonurine in various rat biological samples.

Biological sample	Mean percentage of metabolites against total counts (%)^a				Total peak areas (counts)^b
	M1	M2	M3	M0 (Parent)
Plasma^c	94.59	0.70	N.D.	4.71	3.30×10^6
Bile^d	80.38	1.84	0.28	17.50	5.49×10^7
Urine^e	77.90	0.21	0.08	21.81	2.79×10^7
Feces^e	N.D.	N.D.	1.73	98.27	1.70×10^7

N.D.: not detected.
aCalculated as (peak area of each compound/total peak areas of all compounds) × 100.
bThe peak areas of parent and each metabolite were obtained under MRM scan mode.
^cThe pooled plasma samples collected from 6 rats after oral dosing of leonurine.
^dThe 0–8 h bile samples collected from 6 bile duct-cannulated rats after oral dosing.
^eThe 0–16 h samples collected from 6 rats after oral dosing.