Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy

Table 1

Total solubility parameter (𝛿) and solubility parameter components (𝛿_𝑑,𝛿_𝑝, 𝛿_ℎ) of Spurr’s and LR-White resin chemical components.

Chemicals	Solubility parameter components (MJ ^1/2 m ^−3/2)			Total 𝛿
	𝛿𝑑	𝛿𝑝	𝛿ℎ	(MJ ^1/2 m ^−3/2)
Spurr’s resin
Nonenyl succinic anhydride	16.7	7.7	6.2	19.4
4-Vinyl-1-cyclohexene diepoxide	14.0	7.0	7.3	17.3
Propylene glycol diglycidyl ether	14.9	9.7	8.5	19.7
Dimethylaminoethanol	14.6	16.5	10.5	24.5
Pure Spurr’s resina	—	—	—	19.0
Acetone^c	15.5	10.4	6.9	19.9
1 Resin : 3 Acetone	—	—	—	19.7
1 Resin : 1 Acetone	—	—	—	19.4
3 Resin : 1 Acetone	—	—	—	19.2
LR-White resin
2,2-Propanediylbis(4,1-phenyleneoxy-2,1ethanediyl); bis(2-methylacrylate)	16.7	3.1	7.0	18.3
2-Propenoic acid, 2-methyl-, dodecyl ester	19.1	1.7	4.9	19.8
Pure LR-White resinb	—	—	—	18.7
Ethanol^c	15.8	8.8	19.4	26.4
1 Resin : 3 Ethanol	—	—	—	24.5
1 Resin : 1 Ethanol	—	—	—	22.5
3 Resin : 1 Ethanol	—	—	—	20.6

a26.0 g nonenyl succinic anhydride, 10.0 g 4-vinyl-1-cyclohexene diepoxide, 6.0 g propylene glycol diglycidyl ether, and 0.4 g dimethylaminoethanol [22].
^bAssuming a concentration of 75% 2,2-propanediylbis(4,1-phenyleneoxy-2,1-ethanediyl), bis(2-methylacrylate) and 25% 2-propenoic acid, 2-methyl-, and dodecyl ester.
^cSenichev and tereshatov [28].