A Broad-Spectrum Antimicrobial Activity of Bacillus subtilis RLID 12.1

Table 3

Preliminary characterization of the cell-free supernatant from RLID 12.1.

Treatment	Concentration	Activity
Enzymes
Control		+
Proteinase K	10 mg/mL	+
Pronase	10 mg/mL	+
Trypsin	10 mg/mL	+
Lipase	10 mg/mL	+
-Amylase	10 mg/mL	+
pH
3.0–10.0		+
Temperature
5 hrs at 37°C		+
3 hrs at 50°C		+
3 hrs at 60°C		+
2 hrs at 70°C		+
1 hr at 80°C		+
1 hr at 90°C		+
1 hr at 100°C		+
40 mins at 121°C		+
Surfactants	1% (v/v)	+
EDTA	2, 5, and 50 mg/mL	+
TCA	10 mg/mL	−
Metals	1 mg/mL	+
Organic solvents	50% (v/v)	+