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The Scientific World Journal
Volume 2014, Article ID 969404, 7 pages
http://dx.doi.org/10.1155/2014/969404
Research Article

Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line

1Department of Internal Medicine, Botucatu School of Medicine, University of São Paulo State (UNESP), 18618-000 Botucatu, SP, Brazil
2Department of Radiology, Medicine School, University of São Paulo, USP, 01246-903 São Paulo, SP, Brazil
3Research Center, AC Camargo Hospital, 01509-900 São Paulo, SP, Brazil
4Department of Clinical Medicine of University of São Paulo State, Rubiao Junior District, s/n, 18618-000 Botucatu, SP, Brazil

Received 8 August 2013; Accepted 19 October 2013; Published 23 January 2014

Academic Editors: M. D. Galigniana and V. Sánchez-Margalet

Copyright © 2014 Nancy Bueno Figueiredo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

It has been well established that estrogen plays an important role in the progression and treatment of breast cancer. However, the role of triiodothyronine (T3) remains controversial. We have previously shown its capacity to stimulate the development of positive estrogen receptor breast carcinoma, induce the expression of genes (PR, TGF-alpha) normally stimulated by estradiol (E2), and suppress genes (TGF-beta) normally inhibited by E2. Since T3 regulates growth hormones, metabolism, and differentiation, it is important to verify its action on other genes normally induced by E2. Therefore, we used DNA microarrays to compare gene expression patterns in MCF-7 breast adenocarcinoma cells treated with E2 and T3. Several genes were modulated by both E2 and T3 in MCF-7 cells (Student’s t-test, ). Specifically, we found eight genes that were differentially expressed after treatment with both E2 and T3, including amphiregulin, fibulin 1, claudin 6, pericentriolar material 1, premature ovarian failure 1B, factor for adipocyte differentiation-104, sterile alpha motif domain containing 9, and likely ortholog of rat vacuole membrane protein 1 (fold change > 2.0, pFDR < 0.05). We confirmed our microarray results by real-time PCR. Our findings reveal that certain genes in MCF-7 cells can be regulated by both E2 and T3.