Enhanced Production and Characterization of a Solvent Stable Amylase from Solvent Tolerant Bacillus tequilensis RG-01: Thermostable and Surfactant Resistant

Figure 11

Effect of different concentration of metal ions (NaCl, CaCl₂, and MgSO₄) on amylase production. Test flasks contained different concentrations (5 mM to 45 mM) of metal ions (NaCl, CaCl₂, and MgSO₄) in the medium. The flasks were inoculated with culture and were incubated at initial pH 7.0, 55°C for 24 h. For enzyme activity, the reaction was assayed by standard assay method. Error bars presented mean values of ±standard deviation of triplicates of three independent experiments.