Enzymatic Activity Analysis and Catalytic Essential Residues Identification of Brucella abortus Malate Dehydrogenase

Table 2

Effect of metal ions on MDH activity of B. abortus.

Metal ions^a	Relative activity (%)^b
None	100
Ni^2+	98
Mn^2+	95
Mg^2+	96
Sn^2+	95
Cr^3+	90
Pb^2+	60
Zn^2+	40
Cu^2+	0

aEach metal ion was added into the reaction buffer at 0.5 mM of the concentration, respectively. None means no metal ion added.
^bMDH activity in the reaction buffer without metallic salts was valued as 100%. Relative activity represented the value which compared to that of no additional metal ion added.