Figure 4: A. communis extracts AM and AD induced autophagy in HepG2 and PLC/PRF/5 cells. (a) HepG2 and (c) PLC/PRF/5 cells were treated with Art, AM, or AD for 24 h or UV for 10 min. Then, cells were stained with 5 μg/mL of acridine orange (AO) for 15 min. The autophagic vacuoles were visualized by fluorescence microscopy. AO staining was quantified in control, UV, Art, AM, and AD treated HepG2 (b) and PLC/PRF/5 (d) cells for different concentrations. Art: artocarpin; AM: methanol extract; AD: dichloromethane fraction; UV: ultraviolet.