Identification, Cloning, and Expression of L-Amino Acid Oxidase from Marine Pseudoalteromonas sp. B3

Figure 1

Detection of a-keto acid production using 2,4-dinitrophenylhydrazine (DNP) [13]. ① positive control: α-keto leucine in MM medium (OD520 = 0.232); ② culture supernatant of strain B3 with substrate L-Leu (OD520 = 0.198); ③ culture supernatant of strain B3 without substrate L-Leu (OD520 = 0.032); ④ boiling-denatured culture supernatant of strain B3 with substrate L-Leu (OD520 = 0.045).