Identification, Cloning, and Expression of L-Amino Acid Oxidase from Marine Pseudoalteromonas sp. B3

Figure 6

SDS-PAGE analysis of the expressed recombinant B3-LAAO in E. coli BL21(DE3). Lane M: prestained protein marker (Thermo, USA); lane 1: cell culture of E. coli BL21(DE3)/pET28b(+)-lao with a 6 h-induction by IPTG; lane 2: cell culture of E. coli BL21(DE3)/pET28b(+)-lao without induction; lane 3: cell culture of E. coli BL21(DE3)/pET20b(+)-lao with a 6 h-induction by IPTG; Lane 4: cell culture of E. coli BL21(DE3)/pET20b(+)-lao without induction.