Identification, Cloning, and Expression of L-Amino Acid Oxidase from Marine Pseudoalteromonas sp. B3

Table 1

Conserved regions of LAAOs from common marine microorganisms used for design of degenerate primers.

Item	Accession no.	Sequences of^a
		Region 1	Region 2
Comamonas testosteroni KF-1	ZP_03541004	58VLGAGLAGM66	333ADWCVCTIP341
Dinoroseobacter shibae DFL 12	ABV95616	59VLGAGLAGM67	337ADWCVCTIP345
Caulobacter sp. K31	YP_001683007	57VLGAGLAGM65	331ADWCVCTIP345
Degenerate primer		ZZ-1: 5′-GTGCTSGGCGCKGGYCT-3′	ZF-1: 5′-GTGCARACGCASYAGTCG-3′

aBoldface letters indicate the specific amino acids actually used in the design of the degenerate primers (forward primer: ZZ-1; reverse primer: ZF-1).