Identification, Cloning, and Expression of L-Amino Acid Oxidase from Marine Pseudoalteromonas sp. B3

Table 4

Detection of the H₂O₂ production using Amplex Red Hydrogen Peroxide/Peroxidase Assay kit.

Reactions	Amount of H2O2 (mM)	Relative activity (%)
Test 1^a	1.84	100
Test 2^b	0.14	7.61
Test 3^c	0.043	2.34

aCulture supernatant of strain B3 with substrate L-Leu; ^bculture supernatant of strain B3 without substrate L-Leu; ^csame as Test 1, except that the culture supernatant of strain B3 was denatured with boiling before addition of substrate L-Leu.