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The Scientific World Journal
Volume 2014 (2014), Article ID 980740, 7 pages
http://dx.doi.org/10.1155/2014/980740
Research Article

Cloning and Characterization of a Flavonol Synthase Gene from Scutellaria baicalensis

1Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea
2Department of Biochemistry and Molecular Biology, Baylor College of Medicine One Baylor Plaza, Houston, TX 77030, USA
3Department of Well-Being Resources, Sunchon National University, 413 Jungangno, Suncheon, Jeollanam-do 540-742, Republic of Korea

Received 24 August 2013; Accepted 24 October 2013; Published 28 January 2014

Academic Editors: J. Jakse and E. Porceddu

Copyright © 2014 Yeon Bok Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Flavonols are the most abundant of all the flavonoids and play pivotal roles in a variety of plants. We isolated a cDNA clone encoding flavonol synthase from Scutellaria baicalensis (SbFLS). The SbFLS cDNA is 1011 bp long, encodes 336 amino acid residues, and belongs to a family of 2-oxoglutarate-dependent dioxygenases. The overall structure of SbFLS is very similar to that of Arabidopsis thaliana anthocyanidin synthase (AtANS), with a β jelly-roll fold surrounded by tens of short and long α-helices. SbFLS was constitutively expressed in the roots, stems, leaves, and flowers, with particularly high expression in the roots and flowers. SbFLS transcript levels in the roots were 376-, 70-, and 2.5-fold higher than in the leaves, stems, and flowers. The myricetin content was significantly higher than that of kaempferol and quercetin. Therefore, we suggest that SbFLS mediates flavonol formation in the different organs of S. baicalensis. Our study may contribute to the knowledge of the role of FLS in S. baicalensis.