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The Scientific World Journal
Volume 2015 (2015), Article ID 265385, 7 pages
Research Article

Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India

1College of Veterinary Sciences & A.H., Central Agricultural University, Selesih, Aizawl, Mizoram 796 014, India
2College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam 781 022, India
3National Research Centre on Pigs (ICAR), Rani, Guwahati, Assam 781 131, India

Received 7 July 2014; Revised 19 October 2014; Accepted 12 November 2014

Academic Editor: Gabriella Cancrini

Copyright © 2015 Sonjoy Kumar Borthakur et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of the present study was to determine the prevalence of Dirofilaria immitis in stray, pet, and working dogs (, 266, and 103, resp.) from Guwahati (Assam) and Aizawl (Mizoram), areas located in two Northeastern States of India. Diagnostic methods applied were microscopy (wet film and Knott’s concentration technique), immunological test (Ag ELISA by SNAP 4Dx ELISA kit), and molecular tools (polymerase chain reaction and sequencing), which evidenced 11.38, 18.03, and 13.93% of positive animals, respectively. No significant differences were observed by area (18.23% versus 17.68%) nor by sex (18.1% versus 17.9%), whereas stray dogs proved more infected than other groups (). ELISA test evidenced an overall 22.69% of occult infections, mainly in working dogs (60%), and molecular techniques detected Dirofilaria (Nochtiella) repens in 4 stray dogs from Guwahati. Characterization of D. immitis isolates for ITS-2 region showed close identity with South Asian isolates.