Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India

Table 2

Comparative efficacy percentage of microscopy, Ag ELISA, and PCR in detecting Dirofilaria immitis infection in dogs.

		Detection methods
Dog category	Number of blood samples tested	Microscopy (Knott’s technique)	Ag ELISA; (SNAP 4Dx test)	PCR
		Numbers of positive (%)	Numbers of positive (%)	Specific primer for D. immitis (%)	Panfilarial primers (%)
Stray	413	69 (16.70%)	107 (25.90%)	88 (21.30%)	92 (88 + 4^*); (22.27%)
Pet	266	16 (6.01%)	24 (9.02%)	17 (6.39%)	17 (6.39%)
Working	103	4 (3.88%)	10 (9.70%)	4 (3.88%)	4 (3.88%)
Grand total	782	89 (11.38%)	141 (18.03%)	109 (13.93%)	113 (14.45%)

Dirofilaria repens.