Decolorization of Anthraquinonic Dyes from Textile Effluent Using Horseradish Peroxidase: Optimization and Kinetic Study

Table 4

Best fit of kinetic parameters obtained for the H₂O₂-mediated oxidation of AV 109 and AB 225 by HRP modeled by ping-pong bi-bi model considering inhibition with substrates and without inhibition.

Kinetic parameters	Dye inhibition	Without dye inhibition	Hydrogen peroxide inhibition	Without hydrogen peroxide inhibition
AV 109
(mMmin^−1)	1.638	0.5178	1.097	1.185
(mM)	0.2374	0.0245	0.3302	0.5373
(mM)	0.0080	/	0.4436	/
(mM)	0.4372	0.6908	0.2356	0.8081
	0.9225	0.6456	0.9902	0.7520
AB 225
(mMmin^−1)	0.9813	0.3266	0.5625	1.006
(mM)	0.2213	0.03464	0.1522	0.6069
(mM)	0.0126	/	0.4767	/
(mM)	0.6685	0.8203	0.4536	0.0723
	0.9602	0.7730	0.9908	0.9067

—initial rate of the reaction, —maximum rate, and —initial concentrations of hydrogen peroxide and dye, respectively, , —Michaelis constants for dye and hydrogen peroxide, respectively, , —inhibition constants for hydrogen peroxide and dye, respectively.