Mechanism of Gene Amplification via Yeast Autonomously Replicating Sequences

Figure 2

PCR amplification of the APS from (a) P. ovata and (b) P. lagopus. (a) The migrating bands for the 5S rRNA sequence PCR amplified from the genomic DNA of P. ovata. Lanes 2 and 4 show the 350 bp band corresponding to the PCR amplified APS. Lanes 1 and 3 served as control. In (b) lane 3 served as control and 350 bp bands in lanes 1 and 2 correspond to the PCR amplified 5S rRNA sequence. M is the 100 bp DNA ladder.

(a)

(b)