Mechanism of Gene Amplification via Yeast Autonomously Replicating Sequences

Figure 3

PCR amplification of the ARS from S. cerevisiae. Lane 1 is the control and in lane 3 428 bp band corresponds to the mol. wt. of the ARS315. Lane 4 represents the expected band of the 367 bp corresponding to ARS606. Bright band of 381 bp representing ARS1512 (lane 6), 281 bp band corresponding to ARS1305 (lane 8), 328 bp band corresponding to ARS1426 (lane 15). Lanes 21–25 represent the reamplified ARS and lane 21 represents ARS315; lane 22, ARS606; lane 23, ARS1305; lane 24, ARS1512; and lane 25, ARS1426. Some primer combinations used did not yield desired products (lanes 2, 5, 7, 9, 10, 12, 13, 14, 17, 18, and 20). M is the 1 kb molecular wt marker.