Research Article

Mechanism of Gene Amplification via Yeast Autonomously Replicating Sequences

Figure 4

PCR amplification of the fragile sites located at the Ste-20 gene locus present on chromosome VIII of S. cerevisiae. Lane 1: band of the mol. wt. 613 bp corresponding to Ste-YF1, lane 2: 529 bp band representing Ste-YF2, lane 3: 531 bp band corresponding to Ste-YF3, lanes 6 and 7: control, and lane 4: band of mol. wt. 487 bp representing Ste-YF4. M is the 100 bp molecular wt marker and L is the 1 kb DNA ladder.