Mechanism of Gene Amplification via Yeast Autonomously Replicating Sequences

Figure 5

PCR amplification of the fragile site regions present within the FRA11F fragile site locus on chromosome 11q13 in humans. Lane 1 has the positive control for PCR amplification. Lane 2 and lane 4: PCR amplicon of 546 bp corresponding to FRAI. Lane 3: band of mol. wt. 689 bp corresponding to FRA II. Lanes 7–10: reamplified FRAI sequence. M is the 1 kb DNA ladder.