Effect of In Vitro Transcorneal Approach of Aceclofenac Eye Drops through Excised Goat, Sheep, and Buffalo Corneas
Table 1
In vitro permeation studies of different concentrations of aceclofenac in aqueous solution through excised goat, sheep, and buffalo corneas.
Conc. % (w/v)
Amount permeated (mg) (120 minutes)
Permeation (%) (120 minutes)
Corneal hydration (%)
Papp cm/sec * 106
Goat
Sheep
Buffalo
Goat
Sheep
Buffalo
Goat
Sheep
Buffalo
Goat
Sheep
Buffalo
0.1
1.006 ± 0.005
0.944 ± 0.002
0.789 ± 0.004
98
94.4
78.90
80.2 ± 0.582
80.7 ± 0.001
80.2 ± 0.68
13.81 ± 0.2
12.06 ± 0.02
10.15 ± 0.1
0.2
1.8 ± 0.0012
1.6 ± 0.003
1.62 ± 0.001
90
80
81
80.5 ± 0.021
80.5 ± 0.245
79 ± 0.366
11.55 ± 0.1
10.27 ± 0.1
10.3 ± 0.8
0.3
2.05 ± 0.004
1.96 ± 0.001
1.87 ± 0.003
68.33
65.5
62.4
80.1 ± 0.187
80.9 ± 0.548
80.4 ± 0.366
9.15 ± 0.02
8.78 ± 0.4
8.42 ± 0.6
0.4
2.43 ± 0.006
2.36 ± 0.002
2.24 ± 0.001
60.75
59
56.0
80.4 ± 0.456
80.1 ± 0.216
77 ± 0.325
7.89 ± 0.8
8.41 ± 0.3
7.24 ± 0.2
0.5
2.73 ± 0.001
2.64 ± 0.005
2.58 ± 0.008
54.6
52.8
51.6
79.2 ± 0.213
81.2 ± 0.658
79.3 ± 0.39
7.24 ± 0.12
7.00 ± 0.2
6.8 ± 0.4
Values are mean ± SE of three corneas in each group. Statistically significant ( < 0.05) determined by one-way ANOVA followed by Dunnett’s test. Note: All the experiments were carried out in triplicates.
