Effect of In Vitro Transcorneal Approach of Aceclofenac Eye Drops through Excised Goat, Sheep, and Buffalo Corneas

Table 2

In vitro permeation studies of 0.5% aqueous solution of aceclofenac from with different preservatives through excised goat, sheep, and buffalo cornea.

Preservative

Amount permeated (mg); (120 minutes)

Permeation (%); (120 minutes)

Corneal hydration (%)

Papp cm/sec * 10^6

Surface tension; (dyne/cm)

Goat

Sheep

Buffalo

Goat

Sheep

Buffalo

Goat

Sheep

Buffalo

Goat

Sheep

Buffalo

Control

2.8 ± 0.002

1.2 ± 0.005

1.1 ± 0.004

56

24

22

79.1 ± 0.411

80.4 ± 0.001

79.2 ± 0.55

9.998 ± 0.86

3.36 ± 0.12

2.99 ± 0.36

69.4

BAC

4.4 ± 0.004

2.8 ± 0.008

1.7 ± 0.005

84

76

34

80.5 ± 0.145

78.5 ± 0.548

80.4 ± 0.312

15.01 ± 0.45

9.60 ± 0.03

4.91 ± 0.21

53.1

MP-PP

4.1 ± 0.003

3.6 ± 0.004

1.4 ± 0.003

82

64

28

80.2 ± 0.021

80.1 ± 0.245

79 ± 0.342

15.09 ± 0.40

8.71 ± 0.05

5.09 ± 0.12

54.7

BA

3.2 ± 0.005

2.4 ± 0.004

1.5 ± 0.009

63

50

30

80.9 ± 0.486

80.1 ± 0.116

79 ± 0.325

8.65 ± 0.21

7.01 ± 0.06

5.26 ± 0.32

64.1

THM

1.6 ± 0.009

1.3 ± 0.007

1.1 ± 0.001

32

26

22

79.2 ± 0.125

80.2 ± 0.658

80.3 ± 0.314

5.23 ± 0.45

3.48 ± 0.12

2.99 ± 0.68

60.8

PMA

1.7 ± 0.001

1.1 ± 0.006

1.0 ± 0.001

34

22

20

80.27 ± 0.129

80.2 ± 0.448

80.69 ± 0.314

6.454 ± 0.65

2.99 ± 0.15

2.55 ± 0.45

65.1

BAK indicates benzalkonium chloride; MP-PP, combination of methyl paraben and propylparaben; BA, benzyl alcohol; THM, thiomersal; PMA, phenyl mercuric acetate; values are mean ± SE of three corneas in each group. Statistically significant ( < 0.05) determined by one-way ANOVA followed by Dunnett’s test.
Note: All the experiments were carried out in triplicates.