The Effects of Tempol on Cyclophosphamide-Induced Oxidative Stress in Rat Micturition Reflexes

Figure 1

Cyclophosphamide- (CYP-) induced cystitis increases reactive oxygen species (ROS)/reactive nitrogen species (RNS) and 3-nitrotyrosine (3-NT) in the urinary bladder and the antioxidant, Tempol, reduces expression. (a) 4 hr and 48 hr CYP-induced cystitis significantly () increased ROS/RNS expression in urothelium and detrusor that was significantly () reduced with Tempol. Basal expression and CYP-induced ROS/RNS expression were significantly () greater in detrusor compared to urothelium. Tempol was without effect on ROS/RNS expression in urothelium and detrusor from control (no CYP) rats. (b) 4 hr and 48 hr CYP-induced cystitis significantly () increased ROS/RNS expression in urinary bladder that was significantly () reduced with Tempol. Tempol was without effect on 3-NT expression in urinary bladder from control (no CYP) rats. . for control and treatment groups.

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