Discovery of Specific Inhibitors for Intestinal E. coli  β-Glucuronidase through In Silico Virtual Screening

Figure 2

Specific inhibition of compounds 7145 and 4041 against eG. Compound 7145 and compound 4041 acquired from ligand docking in virtual screening were evaluated based on their selective inhibition for recombinant eG versus hG. 10 M of compound 7145, compound 4041, saccharolactone, and 10% DMSO (control) was incubated with purified eG (□) and hG (■), respectively. G activity was determined by hydrolysis of the pNPG substrate. Error bars represent SD; .