Induced Resistance in Solanum lycopersicum by Algal Elicitor Extracted from Sargassum fusiforme

Figure 5

AP induced resistance reaction in tomato plants. (a) Solanum lycopersicum plants were sprayed with AP 1%, AP 10%, and H₂O (as a negative control) or hyphal wall components (HWC) of P. infestans (as a positive control), and 90 min later the luminol derivative L-012 (0.5 mM L-012 in 10 mM MOPS-KOH (pH 7.4)) was infiltrated to the abaxial surface of the leaves using a needleless syringe. Chemiluminescence was monitored using a photon image processor equipped with a sensitive CCD camera in a dark chamber at 20°C. Representative graphs were taken 90 mpt. (b) Data were quantified using the U7501 program. Shown data are the average of three repeated experiments. Bars with different letters are significantly different at (). (c) S. lycopersicum leaves were infiltrated through the abaxial surface using a needleless syringe with AP 10% and H₂O (as a negative control) or HWC (0.5 mg/mL) (as a positive control) and incubated for 2 or 4 days and then visualized for HR-like cell death using trypan blue staining method as described in Section 2.

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