The Scientific World Journal
Volume 2015, Article ID 956235, 7 pages
http://dx.doi.org/10.1155/2015/956235
Royal Jelly and Its Dual Role in TNBS Colitis in Mice
1Department of Pharmacology, Faculty of Medical Sciences, University of Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil
2Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil
3Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil
4Department of Biological Sciences, Federal University of Goiás (UFG), Regional Catalão, 75704-020 Catalão, GO, Brazil
Received 28 July 2014; Revised 15 October 2014; Accepted 22 October 2014
Academic Editor: Inaya Hajj Hussein
Copyright © 2015 Luis Paulo Manzo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Royal Jelly (RJ) is widely consumed in diets throughout the world due to its beneficial effects: antioxidant, antitumor and anti-inflammatory. We have investigated the role of RJ in the development of TNBS colitis in mice. Colitis was induced by a rectal instillation of TNBS at 0.1 mL per mouse. Intestine samples of the animals orally treated with RJ (100, 150, and 200 mg/kg) were collected for antioxidant assays (GSH and GSH-Px), proinflammatory protein quantification (COX-2 and NF-κB), and histological analyses. RJ 100 mg/kg maintained GSH levels and increased the activity of GSH-Px, downregulated key inflammatory mediators (COX-2 and NF-κB), and decreased the lesions caused by TNBS as shown by the histological analyses. In conclusion, RJ showed anti-inflammatory and antioxidant properties in experimental colitis, resulting in the amelioration of the macroscopic and histological analyses. These results corroborate with the RJ supplementation in diets.