Use of Selected Essential Oils to Control Aflatoxin Contaminated Stored Cashew and Detection of Aflatoxin Biosynthesis Gene

Table 1

Details of the target genes, primer sequences, and expected product length in base pairs (bp) for PCR.

Primer pair	Gene	Primer sequence (5′ → 3′)	PCR; product; size (bp)	Reference
Nor-1F	aflD (nor-1)^a	ACCGCTACGCCGGCACTCTCGGCAC	400
Nor-1R		GTT GGCCGCCAGCTTCGACACTCCG
Ver-1F	aflM (ver-1)	GCCGCAGGCCGCGGAGAAAGTGGT	537
Ver-1R		GGGGATATACTCCCGCGACACAGCC		[14]
Omt-1F	aflP (omt-1)	GTGGACGGACCTAGTCCGACATCAC	797
Omt-1R		GTCGGCGCCACGCACTGGGTTGGGG
aflR-F	aflR	TATCTCCCCCCGGGCATCTCCCGG	1032
aflR-R		GTCGGCGCCACGCACTGGGTTGGGG

Aflatoxin biosynthetic genes are named as proposed by [15]; old names are reported in brackets.