Effects of Droplet-Vitrification Cryopreservation Based on Physiological and Antioxidant Enzyme Activities of Brassidium Shooting Star Orchid

Figure 4

Scanning electron microscopic of stock culture, cryopreserved and noncryopreserved PLBs. (a) stock culture PLB with dense stomata on its surface (arrows), (b) higher magnification of stomata in stock culture PLB, (c) cryopreserved PLB showing damage, (d) higher magnification of cryopreserved PLB showing stomata and damage and undamaged cells, (e) noncryopreserved PLB shrunk, and (f) higher magnification showing stomata of noncryopreserved PLB (dg: damage cell; gc; guard cell; ud: undamaged cell).

(a)

(b)

(c)

(d)

(e)

(f)