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The Scientific World Journal
Volume 2016 (2016), Article ID 1875865, 5 pages
http://dx.doi.org/10.1155/2016/1875865
Research Article

Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India

1Department of Biotechnology, Government Degree College Baramulla, Kashmir 193101, India
2Department of Molecular Biology and Biotechnology, Tezpur University, Assam, India
3Department of Biotechnology, The New College, Chennai, India

Received 24 January 2016; Revised 16 May 2016; Accepted 26 May 2016

Academic Editor: Wen-Jun Li

Copyright © 2016 Abdul Rouf Mir et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study is to probe the pattern of antibiotic resistance against aminoglycosides and its mechanism in E. coli obtained from patients from Chennai, India. Isolation and identification of pathogens were done on MacConkey agar. Antimicrobial sensitivity testing was done by disc diffusion test. The identification of genes encoding aminoglycoside modifying enzymes was done by Polymerase Chain Reaction (PCR). Out of 98 isolates, 71 (72.45%) isolates were identified as E. coli and the remaining 27 (27.55%) as other bacteria. Disc diffusion method results showed a resistance level of 72.15% for streptomycin, 73.4% for gentamicin, 63.26% for neomycin, 57.14% for tobramycin, 47.9% for netilmicin, and 8.16% for amikacin in E. coli. PCR screening showed the presence of four genes, namely, rrs, aacC2, aacA-aphD, and aphA3, in their plasmid DNA. The results point towards the novel mechanism of drug resistance in E. coli from UTI patients in India as they confirm the presence of genes encoding enzymes that cause resistance to aminoglycoside drugs. This could be an alarm for drug prescription to UTI patients.