Nod2-Nodosome in a Cell-Free System: Implications in Pathogenesis and Drug Discovery for Blau Syndrome and Early-Onset Sarcoidosis

Table 1

Oligonucleotide sequences for plasmid construction.

Primer name	Primer sequence
S1-Nod2_F	5′-CCACCCACCACCACCAATGGGGGAAGAGGGTGGTTCAG-3′
Nod2-T1(F)_R	5′-TCCAGCACTAGCTCCAGAAAGCAAGAGTCTGGTGTCCCT-3′
Nod2-T1(CARDs)_R	5′-TCCAGCACTAGCTCCAGACTTGCATGTGGCAGCTTCCA-3′
S1-RICK_F	5′-CCACCCACCACCACCAATGAACGGGGAGGCCATCTG-3′
RICK-T1(F)_R	5′-TCCAGCACTAGCTCCAGACATGCTTTTATTTTGAAGTAAATTTA-3′
S1-RICK(CARD)_F	5′-CCACCCACCACCACCAATGCTGCAGCCTGGTATAGCCC-3′
attB1-S1	5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCCACCCACCACCACCAATG-3′
attB2-T1	5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCTCCAGCACTAGCTCCAGA-3′
Nod2-R334W_F	5′-TTCCCATTCAGCTGCTGGCAGCTGCAGTGC-3′
Nod2-R334W_R	5′-GCACTGCAGCTGCCAGCAGCTGAATGGGAA-3′
Nod2-N670K_F	5′-GCCGAGCCGCACAAACTTCAGATCACAGCA-3′
Nod2-N670K_R	5′-TGCTGTGATCTGAAGTTTGTGCGGCTCGGC-3′

Underline indicates S1 or T1 sequence.
Bold indicates Kozak consensus sequence.
Italic indicates mutation codons for specific amino acids.