Elevation of Glucose 6-Phosphate Dehydrogenase Activity Induced by Amplified Insulin Response in Low Glutathione Levels in Rat Liver

Table 2

Effect of diet on concentration of glutathione (reduced and oxidized forms), metallothionein (MT), and -glutamylcysteine synthetase (-GCS) activity in rat liver.

Diet	GSH (mol/g liver)	GSSG (nmol/g liver)	GSH/GSSG	MT (nmol Hg/g liver)	-GCS (mU/mg protein)
10% SPI	1.2 ± 0.2	53 ± 14	22	244 ± 70	9.2 ± 2.4
10% SPI + cystine	5.9 ± 0.4	43 ± 26	137	66 ± 14	5.4 ± 0.9
10% SPI + methionine	7.0 ± 0.6	39 ± 10	179	104 ± 31	6.7 ± 1.0

Values are mean ± SD of 6 rats fed on respective diets for 3 weeks.
Activity was expressed as mU/mg protein. Amount of mRNA was expressed as relative intensity. Different letters in the same column denote significant difference ().