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The Scientific World Journal
Volume 2016, Article ID 8404092, 6 pages
http://dx.doi.org/10.1155/2016/8404092
Research Article

Fast and Sensitive Method for Determination of Domoic Acid in Mussel Tissue

1Department of Environmental Sciences, Informatics and Statistics, Ca’ Foscari University of Venice, Via Torino 155, Mestre, 30170 Venice, Italy
2Institute for the Dynamics of Environmental Processes, CNR, Via Torino 155, Mestre, 30170 Venice, Italy

Received 2 December 2015; Accepted 4 January 2016

Academic Editor: Núria Fontanals

Copyright © 2016 Elena Barbaro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Domoic acid (DA), a neurotoxic amino acid produced by diatoms, is the main cause of amnesic shellfish poisoning (ASP). In this work, we propose a very simple and fast analytical method to determine DA in mussel tissue. The method consists of two consecutive extractions and requires no purification steps, due to a reduction of the extraction of the interfering species and the application of very sensitive and selective HILIC-MS/MS method. The procedural method was validated through the estimation of trueness, extract yield, precision, detection, and quantification limits of analytical method. The sample preparation was also evaluated through qualitative and quantitative evaluations of the matrix effect. These evaluations were conducted both on the DA-free matrix spiked with known DA concentration and on the reference certified material (RCM). We developed a very selective LC-MS/MS method with a very low value of method detection limit (9 ng g−1) without cleanup steps.