Assessment of Cytotoxic Activity of Rosemary (<i>Rosmarinus officinalis</i> L.), Turmeric (<i>Curcuma longa</i> L.), and Ginger (<i>Zingiber officinale</i> R.) Essential Oils in Cervical Cancer Cells (HeLa)

<div>Morphological changes in HeLa cells treated with<i> R. officinalis</i> (REO),<i> C. longa</i> (CEO), and<i> Z. officinale</i> (GEO) essential oils and dyed with Giemsa. (A) Positive control: cell population treated with DMSO; (B) negative control: cell population without influence of treatment; (C–E) cells treated with 31.12; 249; and 1992 <i>μ</i>g/mL of REO; (F–H) cells treated with 32.81; 262; and 2100 <i>μ</i>g/mL of CEO; (I–K) cells treated with 30.12; 241; and 1928 <i>μ</i>g/mL of GEO. Cell density 1 × 10<sup>6</sup> cells/mL. Images taken at 40x magnification. (<svg height="6.27169pt" id="M30" style="vertical-align:-0.04980993pt" version="1.1" viewbox="-0.0498162 -6.22188 12.5482 6.27169" width="12.5482pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M901 255C861 290 790 361 716 442L694 427L796 280H69V230H796L694 83L716 68C790 149 861 220 901 255Z" id="g117-148"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="954" vert-adv-y="954"></glyph.data></g></svg> cell membrane protrusions (“blebs”); ▸ chromatin condensation; <svg height="6.27169pt" id="M31" style="vertical-align:-0.04980993pt" version="1.1" viewbox="-0.0498162 -6.22188 18.3548 6.27169" width="18.3548pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M567 230V280H69V230H567Z" id="g117-149"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="636" vert-adv-y="636"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,5.776,0)"><path d="M901 255C861 290 790 361 716 442L694 427L796 280H69V230H796L694 83L716 68C790 149 861 220 901 255Z" id="g117-148"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="954" vert-adv-y="954"></glyph.data></g></svg> cell content leakage and formation of apoptotic bodies.)</div>

The Scientific World Journal

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Figure 2

Figure 2: Assessment of Cytotoxic Activity of Rosemary (<i>Rosmarinus officinalis</i> L.), Turmeric (<i>Curcuma longa</i> L.), and Ginger (<i>Zingiber officinale</i> R.) Essential Oils in Cervical Cancer Cells (HeLa) 

Figure 2 | Assessment of Cytotoxic Activity of Rosemary (Rosmarinus officinalis L.), Turmeric (Curcuma longa L.), and Ginger (Zingiber officinale R.) Essential Oils in Cervical Cancer Cells (HeLa) 