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The Scientific World Journal
Volume 2017, Article ID 8367042, 8 pages
Research Article

Analgesic, Anti-Inflammatory, and Antioxidant Activities of Byrsonima duckeana W. R. Anderson (Malpighiaceae)

1Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil
2Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil
3Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, AM, Brazil
4Center of Biological and Health Sciences, Pontifical Catholic University of Paraná, Curitiba, PR, Brazil
5Department of Chemistry, Federal University of Amazonas, Manaus, AM, Brazil
6DeMpSter Mass Spectrometry Group, Amazonas State University, Manaus, AM, Brazil

Correspondence should be addressed to Fernanda Guilhon-Simplicio; rb.ude.mafu@oicilpmis_nohliug

Received 22 December 2016; Accepted 9 February 2017; Published 6 March 2017

Academic Editor: Valdir Cechinel Filho

Copyright © 2017 Maria Christina dos Santos Verdam et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. Byrsonima is a promising neotropical genus, rich in flavonoids and triterpenes, with several proven pharmacological properties. Nevertheless, Byrsonima duckeana W. R. Anderson is an Amazonian species almost not studied. Objective. To assess the antioxidant, anti-inflammatory, and analgesic activities of Byrsonima duckeana leaves. Materials and Methods. We analyzed an ethanol extract and its fractions for polyphenol content and UHPLC-MS/MS, phosphomolybdenum, DPPH, TBARS antioxidant tests, formalin-induced pain, carrageenan-induced peritonitis, acetic acid-induced abdominal writhings, and hot plate assays. Results. All the samples showed high polyphenol content and antioxidant capacity in the phosphomolybdenum, DPPH, and TBARS tests. We identified ethyl gallate, quinic acid, gallic acid, catechin, epicatechin, quercetrin, and quercetin in the samples. B. duckeana was able to reduce leukocyte migration in the carrageenan-induced peritonitis by 43% and the licking time in the formalin test by 57%. In the acetic acid-induced writhing test, the chloroform (FCL) and ethyl acetate (FEA) fractions were the most active samples. FEA was selected for the hot plate test, where all the dosages tested (5, 50, and 200 mg·kg−1) showed significant analgesic activity. Conclusion. B. duckeana has interesting analgesic and antioxidant activities, due to its high phenolic content, especially phenolic acids.